Abstract: Activated B cells, pulsed with tumor-lysates, were examined for their potential to induce tumorspecific CD4+ T cells in vivo.MH2 cells, which are CD4+ T cells and can also recognize purified protein derivative from mycobacterium tuberculosis (PPD), showed a proliferative response in the presence of both syngeneic activated B cells and PPD in a mayor histocompatibility complex class II-restricted manner.Tor B cells to function as efficient antigen presenting cells, they need to be activated.Both irradiation and several inhibitors for antigen-processing were observed to block the antigen-presenting ability of activated B cells.Based in these findings, the possibility of anti-tumor vaccination with activated B cells was thus investigated.The spleen cells from mice, which were immunized with activated B cells pulsed with B16 melanoma-lysates, produced a higher level of interferon (IFN)-y than those from mice, which were immunized with either non-pulsed activated B cells or the tumor-lysates alone, after in vitro restimulation.This IFN-y production was also dependent on the CD4+ T cells.Moreover, the splenic CD4+ T cells in such mice were suggested to increase their ability to generate B16 melanoma-specific cytotoxic T lymphocytes after in vitro restimulation.Even more importantly, immunization with B16 melanoma lysate-pulsed activated B cells elicited a protective immunity against B16 melanoma at rechallenge.Collectively, these results indicate that an anti-tumor effect could be induced by immunization with activated B cells, pulsed with the tumor-lysates, by eliciting tumor-specific IFN-y producing CD4+ T cells in vivo.

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{{Explor lien
   |wiki=    Sante
   |area=    ChloroquineV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     ISTEX:D5212E2101C0EDC9D79B570C1AF53B451E20F5FD
   |texte=   Vaccination with Activated B Cells Pulsed with Tumor-Lysates can Induce Tumor-Specific CD4+ T cells in vivo
}}